sgrna-design-guide
Three-tiered sgRNA design guide using validated Addgene sequences, CRISPick pre-computed datasets, or de novo design rules for CRISPR experiments
npx skills add BioTender-max/awesome-bio-agent-skills --skill sgrna-design-guide --agent claude-code
Same command for any agent — swap --agent for codex, cursor, copilot.
Weekly change comes from our own snapshots, not the repository page — it measures attention, not adoption.
# sgRNA Design Guide: Three-Tiered Approach --- ## Metadata **Short Description**: Comprehensive guide for finding or designing sgRNAs using validated sequences, CRISPick datasets, or de novo design tools. **Authors**: Ohagent Team **Version**: 1.0 **Last Updated**: November 2025 **License**: CC BY 4.0 **Commercial Use**: Allowed ## Citations and Acknowledgments ### If you use validated sgRNAs from our database (Option 1): - **Database Source**: Addgene (https://www.addgene.org) - **Citation**: Always cite the original publication associated with each sgRNA using the PubMed ID provided in the database - **Acknowledgment**: "Validated sgRNA sequences obtained from Addgene (https://www.addgene.org/crispr/reference/grna-sequence/)" ### If you use CRISPick designs (Option 2): - **Acknowledgment Statement**: "Guide designs provided by the CRISPick web tool of the GPP at the Broad Institute" - **Citation for Cas9 designs (SpCas9, SaCas9)**: Sanson KR, et al. Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities. Nat Commun. 2018;9(1):5416. PMID: 30575746 - **Citation for Cas12a designs (AsCas12a, enAsCas12a)**: DeWeirdt PC, et al. Optimization of AsCas12a for combi
- Metadata
- Citations and Acknowledgments
- If you use validated sgRNAs from our database (Option 1):
- If you use CRISPick designs (Option 2):
- Overview
- Key Concepts
- Validated sgRNA Databases (Tier 1)
- Computational sgRNA Scoring (Tier 2)
- Off-Target Stringency and PAM Compatibility
- De Novo Design Rules (Tier 3)
- Decision Framework
- Best Practices
- Common Pitfalls
- Option 1: Search Validated sgRNA Sequences (Recommended First)
Search the download links file grep "9606_GRCh38_SpyoCas9_CRISPRko" resource/CRISPick_download_links.txt Or for mouse grep "10090_GRCm38_SpyoCas9_CRISPRko" resource/CRISPick_download_links.txt Copy the URL from download_links.txt, then: wget [PASTE_URL_HERE] Extract the file gunzip sgRNA_design_*.txt.gz Download human GRCh38 SpCas9 knockout dataset wget https://portals.broadinstitute.org/gppx/public/sgrna_design/api/downloads/\
What does the sgrna-design-guide skill do?
Three-tiered sgRNA design guide using validated Addgene sequences, CRISPick pre-computed datasets, or de novo design rules for CRISPR experiments
How do I install it?
Run `npx skills add BioTender-max/awesome-bio-agent-skills --skill sgrna-design-guide --agent claude-code` — it drops the skill into your project so the agent can pick it up. Swap the --agent value for codex, cursor or copilot if you use one of those.
Where does this skill come from?
From BioTender-max/awesome-bio-agent-skills, a repository with 135 stars. We read it straight from the repository tree rather than a submitted listing, so what you see here is what is actually published.
Is a popular skill a good skill?
Not necessarily. Stars measure attention, not adoption — a repository can trend for a week and be abandoned. That is why we show the weekly change from our own snapshots next to the total, instead of a single flattering number.