bio-restriction-enzyme-selection
Select restriction enzymes by criteria using Biopython Bio.Restriction. Find enzymes that cut once, don't cut, produce specific overhangs, are commercially available, or have compatible ends for cloning. Use when selecting restriction enzymes for cloning or analysis.
npx skills add BioTender-max/awesome-bio-agent-skills --skill enzyme-selection --agent claude-code
Same command for any agent — swap --agent for codex, cursor, copilot.
Weekly change comes from our own snapshots, not the repository page — it measures attention, not adoption.
## Version Compatibility Reference examples tested with: BioPython 1.83+ Before using code patterns, verify installed versions match. If versions differ: - Python: `pip show <package>` then `help(module.function)` to check signatures If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying. # Enzyme Selection **"Find enzymes that cut my sequence once"** → Search restriction enzyme databases by cut frequency, overhang type, commercial availability, or compatible ends for cloning. - Python: `Bio.Restriction.Analysis(rb, seq)` with `RestrictionBatch` filters ## Find Enzymes by Cut Frequency ```python from Bio import SeqIO from Bio.Restriction import Analysis, CommOnly, AllEnzymes record = SeqIO.read('sequence.fasta', 'fasta') seq = record.seq analysis = Analysis(CommOnly, seq) # Enzymes that cut exactly once (good for linearization) once_cutters = analysis.once_cutters() # Enzymes that cut exactly twice (good for excision) twice_cutters = analysis.twice_cutters() # Enzymes that don't cut (good for cloning insert) non_cutters = analysis.only_dont_cut() # All enzymes that cut (any number
- Version Compatibility
- Find Enzymes by Cut Frequency
- Find Non-Cutters for Insert
- Find Compatible Enzyme Pairs
- Filter by Overhang Type
- Filter by Recognition Site Length
- Find Enzymes with Specific Overhang
- Find Unique Cutters in Multiple Sequences
- Select Enzymes for Directional Cloning
- Check Commercial Availability
- Find Isoschizomers
- Check Methylation Sensitivity
- Type IIS Enzymes for Golden Gate Cloning
- Enzyme Properties Reference
What does the bio-restriction-enzyme-selection skill do?
Select restriction enzymes by criteria using Biopython Bio.Restriction. Find enzymes that cut once, don't cut, produce specific overhangs, are commercially available, or have compatible ends for cloning. Use when selecting restriction enzymes for cloning or analysis.
How do I install it?
Run `npx skills add BioTender-max/awesome-bio-agent-skills --skill enzyme-selection --agent claude-code` — it drops the skill into your project so the agent can pick it up. Swap the --agent value for codex, cursor or copilot if you use one of those.
Where does this skill come from?
From BioTender-max/awesome-bio-agent-skills, a repository with 135 stars. We read it straight from the repository tree rather than a submitted listing, so what you see here is what is actually published.
Is a popular skill a good skill?
Not necessarily. Stars measure attention, not adoption — a repository can trend for a week and be abandoned. That is why we show the weekly change from our own snapshots next to the total, instead of a single flattering number.