crispr-designer
Designs guide RNA (gRNA) sequences for CRISPR-Cas9 editing, including off-target analysis. Use when a user needs to edit a gene or asks for gRNA sequences.
npx skills add majiayu000/claude-skill-registry --skill crispr-designer-mdbabumiamssm-llms-universal-life --agent claude-code
Same command for any agent — swap --agent for codex, cursor, copilot.
Weekly change comes from our own snapshots, not the repository page — it measures attention, not adoption.
# CRISPR gRNA Designer This skill designs high-efficiency guide RNAs for gene editing experiments. ## When to use this skill - When a user provides a gene name (e.g., "TP53"), Ensembl ID, or DNA sequence. - When the user wants to perform "knockout", "activation" (CRISPRa), or "interference" (CRISPRi). - When specificity and off-target minimization are requested. ## How to use it 1. **Identify Target Locus:** - Resolve the gene name to the current reference genome (GRCh38 for humans). - Identify functional domains (exons) that are constitutively expressed. 2. **Design gRNAs:** - Select 20nt targets adjacent to NGG PAM sites. - Prioritize 5' constitutive exons for knockouts to ensure early truncation. 3. **Score Candidates:** - Calculate **On-Target Efficiency** (e.g., Rule Set 2 score). - Calculate **Off-Target Specificity** (CFD score) by searching the whole genome for mismatches. 4. **Output Table:** - Return a markdown table with: Sequence, PAM, On-Target Score, Off-Target Score, and Genomic Location. - Recommend the top 3 guides.
- When to use this skill
- How to use it
What does the crispr-designer skill do?
Designs guide RNA (gRNA) sequences for CRISPR-Cas9 editing, including off-target analysis. Use when a user needs to edit a gene or asks for gRNA sequences.
How do I install it?
Run `npx skills add majiayu000/claude-skill-registry --skill crispr-designer-mdbabumiamssm-llms-universal-life --agent claude-code` — it drops the skill into your project so the agent can pick it up. Swap the --agent value for codex, cursor or copilot if you use one of those.
Where does this skill come from?
From majiayu000/claude-skill-registry, a repository with 534 stars. We read it straight from the repository tree rather than a submitted listing, so what you see here is what is actually published.
Is a popular skill a good skill?
Not necessarily. Stars measure attention, not adoption — a repository can trend for a week and be abandoned. That is why we show the weekly change from our own snapshots next to the total, instead of a single flattering number.