cell-free-expression
Guidance for cell-free protein synthesis (CFPS) optimization. Use when: (1) Planning CFPS experiments, (2) Troubleshooting low yield or aggregation, (3) Optimizing DNA template design for CFPS, (4) Expressing difficult proteins (disulfide-rich, toxic, membrane).
npx skills add FreedomIntelligence/OpenClaw-Medical-Skills --skill cell-free-expression --agent claude-code
Same command for any agent — swap --agent for codex, cursor, copilot.
Weekly change comes from our own snapshots, not the repository page — it measures attention, not adoption.
# Cell-Free Protein Synthesis (CFPS) ## System Selection Guide | System | Best For | Yield | PTMs | Disulfides | Cost | |--------|----------|-------|------|------------|------| | **E. coli extract** | Rapid prototyping, prokaryotic proteins | High (100-400 μg/mL) | None | Poor (reducing) | Low | | **E. coli PURE** | Defined conditions, unnatural AAs | Medium (50-150 μg/mL) | None | Controllable | High | | **Wheat germ** | Eukaryotic proteins, membrane proteins | High (100-500 μg/mL) | Limited | Moderate | Medium | | **Rabbit reticulocyte** | Mammalian proteins, post-translational studies | Low (10-50 μg/mL) | Some | Poor | High | | **Insect (Sf21)** | Glycoproteins, complex folds | Medium (50-100 μg/mL) | Glycosylation | Good | High | | **HeLa/CHO** | Native mammalian proteins | Low (10-50 μg/mL) | Full mammalian | Good | Very High | --- ## CFPS Troubleshooting Matrix | Problem | Likely Causes | Design Fix | Reagent Fix | |---------|---------------|------------|-------------| | **No expression** | Rare codons at N-terminus, poor RBS | Codon optimize first 30 codons | Use BL21-CodonPlus extract | | **Low yield** | Strong mRNA secondary structure, template issues | Optimize 5' UTR (Δ
- System Selection Guide
- CFPS Troubleshooting Matrix
- Codon Optimization for CFPS
- Codons to Avoid in E. coli CFPS
- Design Rules
- When to Use Rare Codons
- mRNA Template Design
- 5' UTR Optimization
- Secondary Structure Targets
- Template Format
- Disulfide Bond Formation
- System Capabilities
- Oxidative Folding Protocol (E. coli extract)
- Disulfide-Rich Protein Tips
What does the cell-free-expression skill do?
Guidance for cell-free protein synthesis (CFPS) optimization. Use when: (1) Planning CFPS experiments, (2) Troubleshooting low yield or aggregation, (3) Optimizing DNA template design for CFPS, (4) Expressing difficult proteins (disulfide-rich, toxic, membrane).
How do I install it?
Run `npx skills add FreedomIntelligence/OpenClaw-Medical-Skills --skill cell-free-expression --agent claude-code` — it drops the skill into your project so the agent can pick it up. Swap the --agent value for codex, cursor or copilot if you use one of those.
Where does this skill come from?
From FreedomIntelligence/OpenClaw-Medical-Skills, a repository with 2,909 stars. We read it straight from the repository tree rather than a submitted listing, so what you see here is what is actually published.
Is a popular skill a good skill?
Not necessarily. Stars measure attention, not adoption — a repository can trend for a week and be abandoned. That is why we show the weekly change from our own snapshots next to the total, instead of a single flattering number.