bio-ribo-seq-ribosome-periodicity
Validate Ribo-seq data quality by checking 3-nucleotide periodicity and calculating P-site offsets. Use when assessing library quality or determining read offsets for downstream analysis.
npx skills add FreedomIntelligence/OpenClaw-Medical-Skills --skill bio-ribo-seq-ribosome-periodicity --agent claude-code
Same command for any agent — swap --agent for codex, cursor, copilot.
Weekly change comes from our own snapshots, not the repository page — it measures attention, not adoption.
## Version Compatibility Reference examples tested with: matplotlib 3.8+, numpy 1.26+, pysam 0.22+, scipy 1.12+ Before using code patterns, verify installed versions match. If versions differ: - Python: `pip show <package>` then `help(module.function)` to check signatures - CLI: `<tool> --version` then `<tool> --help` to confirm flags If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying. # Ribosome Periodicity Analysis **"Check if my Ribo-seq data shows triplet periodicity"** → Validate Ribo-seq library quality by verifying 3-nucleotide translocation patterns and calculating P-site offsets from metagene profiles. - Python: `plastid` for P-site offset calculation and metagene analysis ## 3-Nucleotide Periodicity **Goal:** Verify that Ribo-seq reads exhibit the expected 3-nucleotide translocation pattern characteristic of active translation. **Approach:** Load P-site mapped reads and compute metagene profiles around start codons to check for triplet periodicity. Ribosomes move 3 nucleotides per codon. Good Ribo-seq data shows strong periodicity: ```python from plastid import BAMGe
- Version Compatibility
- 3-Nucleotide Periodicity
- Calculate P-site Offset
- Metagene Plots
- Assess by Read Length
- P-site Offset Table
- Validate with RiboCode
- Related Skills
RiboCode includes periodicity analysis RiboCode_onestep \ Check output for periodicity plots
What does the bio-ribo-seq-ribosome-periodicity skill do?
Validate Ribo-seq data quality by checking 3-nucleotide periodicity and calculating P-site offsets. Use when assessing library quality or determining read offsets for downstream analysis.
How do I install it?
Run `npx skills add FreedomIntelligence/OpenClaw-Medical-Skills --skill bio-ribo-seq-ribosome-periodicity --agent claude-code` — it drops the skill into your project so the agent can pick it up. Swap the --agent value for codex, cursor or copilot if you use one of those.
Where does this skill come from?
From FreedomIntelligence/OpenClaw-Medical-Skills, a repository with 2,909 stars. We read it straight from the repository tree rather than a submitted listing, so what you see here is what is actually published.
Is a popular skill a good skill?
Not necessarily. Stars measure attention, not adoption — a repository can trend for a week and be abandoned. That is why we show the weekly change from our own snapshots next to the total, instead of a single flattering number.