bio-genome-engineering-prime-editing-design
Design pegRNAs for prime editing using PrimeDesign algorithms. Generate spacer, PBS, and RT template sequences for precise genomic modifications without double-strand breaks. Use when designing prime editing experiments for precise insertions, deletions, or point mutations.
npx skills add FreedomIntelligence/OpenClaw-Medical-Skills --skill bio-genome-engineering-prime-editing-design --agent claude-code
Same command for any agent — swap --agent for codex, cursor, copilot.
Weekly change comes from our own snapshots, not the repository page — it measures attention, not adoption.
## Version Compatibility Reference examples tested with: BioPython 1.83+ Before using code patterns, verify installed versions match. If versions differ: - Python: `pip show <package>` then `help(module.function)` to check signatures If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying. # Prime Editing Design **"Design a prime editing guide for my point mutation"** → Generate pegRNA sequences (spacer, scaffold, RT template, PBS) for precise genomic modifications without double-strand breaks, optimizing PBS length and RT template for editing efficiency. - Python: PrimeDesign algorithms with `Bio.Seq` for sequence handling ## pegRNA Structure ``` pegRNA components: 1. Spacer (20nt) - guides Cas9 to target site 2. Scaffold - Cas9 binding sequence 3. RT template - encodes the desired edit 4. PBS (primer binding site) - anneals to nicked strand Spacer (20nt) Scaffold RT template PBS 5'─[NNNNNNNNNNNNNNNNNNNN]─[scaffold]─[edit]─────[PBS]─3' ``` ## Design pegRNA for Point Mutation ```python from Bio.Seq import Seq def design_pegrna_substitution(target_seq, edit_pos, new_base, pbs_length
- Version Compatibility
- pegRNA Structure
- Design pegRNA for Point Mutation
- PBS Length Optimization
- RT Template Design
- PE3 Nicking Guide Design
- Complete pegRNA Assembly
- Related Skills
What does the bio-genome-engineering-prime-editing-design skill do?
Design pegRNAs for prime editing using PrimeDesign algorithms. Generate spacer, PBS, and RT template sequences for precise genomic modifications without double-strand breaks. Use when designing prime editing experiments for precise insertions, deletions, or point mutations.
How do I install it?
Run `npx skills add FreedomIntelligence/OpenClaw-Medical-Skills --skill bio-genome-engineering-prime-editing-design --agent claude-code` — it drops the skill into your project so the agent can pick it up. Swap the --agent value for codex, cursor or copilot if you use one of those.
Where does this skill come from?
From FreedomIntelligence/OpenClaw-Medical-Skills, a repository with 2,909 stars. We read it straight from the repository tree rather than a submitted listing, so what you see here is what is actually published.
Is a popular skill a good skill?
Not necessarily. Stars measure attention, not adoption — a repository can trend for a week and be abandoned. That is why we show the weekly change from our own snapshots next to the total, instead of a single flattering number.