bio-genome-engineering-hdr-template-design
Design homology-directed repair donor templates for CRISPR knock-ins using primer3-py. Create ssODN, dsDNA, or plasmid templates with optimized homology arms. Use when designing donor templates for precise insertions, tagging, or allele replacement.
npx skills add FreedomIntelligence/OpenClaw-Medical-Skills --skill bio-genome-engineering-hdr-template-design --agent claude-code
Same command for any agent — swap --agent for codex, cursor, copilot.
Weekly change comes from our own snapshots, not the repository page — it measures attention, not adoption.
## Version Compatibility Reference examples tested with: BioPython 1.83+, primer3-py 2.0+ Before using code patterns, verify installed versions match. If versions differ: - Python: `pip show <package>` then `help(module.function)` to check signatures If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying. # HDR Template Design **"Design a donor template for my CRISPR knock-in"** → Create homology-directed repair templates (ssODN, dsDNA, or plasmid) with optimized homology arm lengths and silent PAM mutations, using primer3 for flanking primer design. - Python: `primer3.bindings.design_primers()` (primer3-py) for primer/arm design, `Bio.Seq` for template construction ## Template Types ``` ssODN (single-stranded oligodeoxynucleotide): - Length: 100-200nt total - Homology arms: 30-60nt each side - Best for: Small insertions (<50bp), point mutations - Delivery: Electroporation with RNP dsDNA (double-stranded DNA): - Length: 500bp - 5kb total - Homology arms: 200-800bp each side - Best for: Larger insertions (tags, reporters) - Delivery: Plasmid or PCR product Plasmid donor: - Homology
- Version Compatibility
- Template Types
- ssODN Design
- Asymmetric Arm Design
- dsDNA Donor Design
- Common Insertions
- PAM Mutation to Prevent Re-cutting
- Related Skills
What does the bio-genome-engineering-hdr-template-design skill do?
Design homology-directed repair donor templates for CRISPR knock-ins using primer3-py. Create ssODN, dsDNA, or plasmid templates with optimized homology arms. Use when designing donor templates for precise insertions, tagging, or allele replacement.
How do I install it?
Run `npx skills add FreedomIntelligence/OpenClaw-Medical-Skills --skill bio-genome-engineering-hdr-template-design --agent claude-code` — it drops the skill into your project so the agent can pick it up. Swap the --agent value for codex, cursor or copilot if you use one of those.
Where does this skill come from?
From FreedomIntelligence/OpenClaw-Medical-Skills, a repository with 2,909 stars. We read it straight from the repository tree rather than a submitted listing, so what you see here is what is actually published.
Is a popular skill a good skill?
Not necessarily. Stars measure attention, not adoption — a repository can trend for a week and be abandoned. That is why we show the weekly change from our own snapshots next to the total, instead of a single flattering number.