binding-characterization
Guidance for SPR and BLI binding characterization experiments. Use when: (1) Planning binding kinetics experiments, (2) Troubleshooting poor/no binding signal, (3) Interpreting kinetic data artifacts, (4) Choosing between SPR vs BLI platforms.
npx skills add FreedomIntelligence/OpenClaw-Medical-Skills --skill binding-characterization --agent claude-code
Same command for any agent — swap --agent for codex, cursor, copilot.
Weekly change comes from our own snapshots, not the repository page — it measures attention, not adoption.
# Binding Characterization: SPR and BLI ## SPR vs BLI Decision Matrix | Factor | Choose SPR | Choose BLI | |--------|------------|------------| | **Sensitivity** | Small molecules, fragments (<500 Da) | Large complexes, antibodies | | **Throughput** | Low-medium (serial) | High (96-well parallel) | | **Sample purity** | Required (clogs fluidics) | Tolerates crude lysates | | **Kinetic resolution** | Higher (better for fast kinetics) | Lower | | **Mass transport** | More sensitive (may distort kon) | Less sensitive | | **Maintenance** | High (fluidics system) | Low (dip-and-read) | | **Sample consumption** | Higher (continuous flow) | Lower | | **Cost per experiment** | Lower chip cost, higher run cost | Higher tip cost, lower run cost | ## Key differences ### SPR (Surface Plasmon Resonance) - **Mechanism**: Detects refractive index changes at gold surface - **Surface**: Gold chip with dextran matrix (CM5, CM7, etc.) - **Flow**: Continuous microfluidics - **Best for**: Small molecules, high-affinity, precise kon/koff ### BLI (Biolayer Interferometry) - **Mechanism**: Measures optical interference pattern shift - **Surface**: Fiber optic biosensor tips (SA, Ni-NTA, AHC) - **Flow**: D
- SPR vs BLI Decision Matrix
- Key differences
- SPR (Surface Plasmon Resonance)
- BLI (Biolayer Interferometry)
- Troubleshooting: Why BLI works but SPR doesn't
- Why SPR works but BLI doesn't
- Mass transport considerations
- Symptoms
- When mass transport matters
- Mitigation strategies
- Nonspecific binding mitigation
- Buffer additives (ranked by effectiveness)
- pH optimization
- Reference subtraction
What does the binding-characterization skill do?
Guidance for SPR and BLI binding characterization experiments. Use when: (1) Planning binding kinetics experiments, (2) Troubleshooting poor/no binding signal, (3) Interpreting kinetic data artifacts, (4) Choosing between SPR vs BLI platforms.
How do I install it?
Run `npx skills add FreedomIntelligence/OpenClaw-Medical-Skills --skill binding-characterization --agent claude-code` — it drops the skill into your project so the agent can pick it up. Swap the --agent value for codex, cursor or copilot if you use one of those.
Where does this skill come from?
From FreedomIntelligence/OpenClaw-Medical-Skills, a repository with 2,909 stars. We read it straight from the repository tree rather than a submitted listing, so what you see here is what is actually published.
Is a popular skill a good skill?
Not necessarily. Stars measure attention, not adoption — a repository can trend for a week and be abandoned. That is why we show the weekly change from our own snapshots next to the total, instead of a single flattering number.